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Strain Name
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NOD.CB17-PrkdcscidIl2rgtm1BcgenIl15tm1(IL15)BcgenFcgr1tm1BcgenFcgr2btm1BcgenFcgr4tm1BcgenFcgr3tm1Bcgen/Bcgen
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Common Name
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B-NDG hIL15, FcγR KO mice
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Background
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B-NDG mice
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Catalog number
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112653
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Aliases
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IL15: IL-15
Fcgr1: CD64, FcgammaRI, IGGHAFC
Fcgr2b: CD32, F630109E10Rik, Fc[g]RII, FcgRII, Fcgr2, Fcgr2a, Fcr-2, Fcr-3, Ly-17, Ly-m20, LyM-1, Lym-1, fcRII
Fcgr4: 4833442P21Rik, CD16-2, FcgRIV, FcgammaRIV, Fcgr3a, Fcrl3
Fcgr3: CD16
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Protein expression analysis
Strain specific FcγRs expression analysis in homozygous B-NDG hIL15 mice and B-NDG hIL15, FcγR KO mice by flow cytometry. Spleens were collected from homozygous B-NDG hIL15 mice and B-NDG hIL15, FcγR KO mice, and analyzed by flow cytometry with four species-specific anti-FcγR antibodies. Mouse FcγRI, FcγRIIb, FcγRIII and FcγRIV were detectable in B-NDG hIL15 mice but not in B-NDG hIL15, FcγR KO mice.
Protein expression analysis
Strain specific IL15 expression analysis in B-NDG mice, homozygous B-NDG hIL15 mice and B-NDG hIL15, FcγR KO mice by ELISA. Serum was collected from male B-NDG mice (9-week-old) , homozygous B-NDG hIL15 mice (9-week-old) and B-NDG hIL15, FcγR KO mice (12-week-old), and analyzed by ELISA with species-specific IL15 antibody. Human IL15 was detectable in homozygous B-NDG hIL15 mice and B-NDG hIL15, FcγR KO mice but not in B-NDG mice. The expression level in B-NDG hIL15, FcγR KO mice was similar to that in B-NDG hIL15 mice.
Analysis of leukocyte subpopulations in blood
Analysis of blood leukocyte subpopulations by flow cytometry. Blood were isolated from male B-NDG hIL15 mice and B-NDG hIL15, FcγR KO mice (n=3). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of granulocytes, monocytes, macrophages and DCs in homozygous B-NDG hIL15, FcγR KO mice were similar to those in the B-NDG hIL15 mice, demonstrating that knock out of FcγRs does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.
Analysis of leukocytes cell subpopulation in spleen
Analysis of spleen leukocyte subpopulations by flow cytometry. Splenocytes were isolated from male B-NDG hIL15 mice and B-NDG hIL15, FcγR KO mice (n=3). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of granulocytes, monocytes, macrophages and DCs in homozygous B-NDG hIL15, FcγR KO mice were similar to those in the B-NDG hIL15 mice, demonstrating that knock out of FcγRs does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
